Mouse Monoclonal Antibody to CD281

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Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.

Product Overview
Entrez GenelD
7096
Aliases
TLR1; TIL; rsc786; TIL. LPRS5
Clone#
3B1D8
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD281 (AA: extra 172-425) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomed Environ Sci. 2016 Nov;29(11):825-828. 2.Transpl Immunol. 2016 Sep;38:60-6.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD281 mAb against human CD281 (AA: extra 172-425) recombinant protein. (Expected MW is 55.1 kDa)
Figure 2:Western blot analysis using CD281 mAb against human CD281 (AA: extra 172-425) recombinant protein. (Expected MW is 55.1 kDa)
Western Blot
Figure 3:Western blot analysis using CD281 mAb against HEK293-6e (1) and CD281 (AA: extra 172-425)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD281 mAb against HEK293-6e (1) and CD281 (AA: extra 172-425)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using CD281 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using CD281 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using CD281 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of THP-1 cells using CD281 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded lung tissues using CD281 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded lung tissues using CD281 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CD281 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CD281 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 32098

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