Mouse Monoclonal Antibody to CD273
Item Information
Catalog #
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Price
Description
PDCD1LG2 (Programmed Cell Death 1 Ligand 2) is a Protein Coding gene. Diseases associated with PDCD1LG2 include Cysticercosis and Testicular Lymphoma. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. An important paralog of this gene is CD274.
Product Overview
Entrez GenelD
80380
Aliases
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Clone#
5F4H10
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human CD273 (AA: 20-220) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Immunother Cancer. 2019 Aug 29;7(1):233. 2,Cancer Sci. 2020 Apr;111(4):1113-1123.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD273 mAb against human CD273 (AA: 20-220) recombinant protein. (Expected MW is 48.6 kDa)
Western Blot
Figure 3:Western blot analysis using CD273 mouse mAb against HepG2 (1), Hela (2),NIH/3T3 (3),PC-12 (4),K562 (5),Jurkat (6),MCF-7 (7), and HEK293-6e (8) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using CD273 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using CD273 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of K562 cells using CD273 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using CD273 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.