Mouse Monoclonal Antibody to CD273
Item Information
Catalog #
Size
Price
Description
PDCD1LG2 (Programmed Cell Death 1 Ligand 2) is a Protein Coding gene. Diseases associated with PDCD1LG2 include Cysticercosis and Testicular Lymphoma. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. An important paralog of this gene is CD274.
Product Overview
Entrez GenelD
80380
Aliases
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Clone#
5H8B11
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CD273 (AA: 20-220) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Immunother Cancer. 2019 Aug 29;7(1):233. 2,Cancer Sci. 2020 Apr;111(4):1113-1123.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/52a9d0c830ee75605416e00fb009aafd49290677ad8f650c5deaa47baf2cd62f.jpg?v=1666705309)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD273 mAb against human CD273 (AA: 20-220) recombinant protein. (Expected MW is 48.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ff63e5c30fcf2bf20ef04aca71ab1de8b80ef44e3a0229e27125edab934381bf.jpg?v=1666705309)
Figure 2:Western blot analysis using CD273 mAb against human CD273 (AA: 20-220) recombinant protein. (Expected MW is 48.6 kDa)
Western Blot
![Figure 3:Western blot analysis using CD273 mAb against HEK293-6e (1) and CD273 (AA: 20-220)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5df97af8ecf2cda1c8e98dba97207d440783a0a4f44abd5494fb58a45713fdd7.jpg?v=1666705309)
Figure 3:Western blot analysis using CD273 mAb against HEK293-6e (1) and CD273 (AA: 20-220)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Hela cells using CD273 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9787acb4c1d4c1f50a5eabdc858df261a81bb7c6662b930a07294b38bf2cc2e9.jpg?v=1666705309)
Figure 4:Flow cytometric analysis of Hela cells using CD273 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using CD273 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0d2e42cfd9560a1fa39f7580430b99dbd9d2313f42e7cd8fc2dc916c8052fd47.jpg?v=1666705309)
Figure 5:Flow cytometric analysis of Jurkat cells using CD273 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of K562 cells using CD273 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/91486c0c38722b511908cbb038c6f2746ab7c7c0e38eee3b17322a94b1d56c34.jpg?v=1666705309)
Figure 6:Flow cytometric analysis of K562 cells using CD273 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.