CD27 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Product Overview
Entrez GenelD
939
Aliases
T14; S152; Tp55; TNFRSF7; S152. LPFS2
Clone#
3B6D3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD27 (AA: extra 20-144) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Blood Cancer J. 2017 Jun 23;7(6):e575.
2.J Int Med Res. 2012;40(1):307-16.
2.J Int Med Res. 2012;40(1):307-16.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD27 mAb against human CD27 (AA: extra 20-144) recombinant protein. (Expected MW is 39.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD27 mAb against HEK293 (1) and CD27 (AA: extra 20-144)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD27 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.