CD268 Primary Antibody
Item Information
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Description
B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of Baff in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular cysteine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Product Overview
Entrez GenelD
115650
Aliases
TNFRSF13C; BAFFR; CVID4; BAFF-R; BROMIX; prolixin
Clone#
5A9B10
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD268 (AA: extra 1-78) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Cancer Ther. 2014 Jun;13(6):1567-77.
2.Mol Cell Biochem. 2011 Nov;357(1-2):21-30.
2.Mol Cell Biochem. 2011 Nov;357(1-2):21-30.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD268 mAb against human CD268 (AA: extra 1-78) recombinant protein. (Expected MW is 34 kDa)
Western Blot
Figure 3:Western blot analysis using CD268 mAb against HEK293 (1) and CD268 (AA: extra 1-78)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Raji cells using CD268 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.