Mouse Monoclonal Antibody to CD258
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a46e3d87c958221c5f1ea31a37759aea107e8ab8146d8d9f9e1012308678074d.jpg?v=1666705266)
![Figure 2:Western blot analysis using CD258 mAb against human CD258 (AA: extra 59-240) recombinant protein. (Expected MW is 46 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a7fcc06e714b0d4502b333faf5504f724e88d5f32079f92e023bb4150fae6b8b.jpg?v=1666705266)
![Figure 3:Western blot analysis using CD258 mAb against HEK293-6e (1) and CD258 (AA: extra 59-240)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5117b034a99869713928e17ea49d0a44099a5fceffadbc184469c409f65746d0.jpg?v=1666705266)
![Figure 4:Immunofluorescence analysis of Hela cells using CD258 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/11d67348bb321dc81eed108c642266a91d9f100a02b426f60ede489f31a3d57c.jpg?v=1666705266)
![Figure 5:Flow cytometric analysis of THP-1 cells using CD258 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a634b50a5f68c116115068f4e16b3bff47611ef9bdeff3d5706f3d211d1faf56.jpg?v=1666705266)