Mouse Monoclonal Antibody to CD226
Item Information
Catalog #
Size
Price
Description
This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
10666
Aliases
PTA1; DNAM1; DNAM-1; TLiSA1
Clone#
4D8E1B4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD226 (AA: extra 19-254) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Immunol Invest. 2018 Apr;47(3):264-278. 2.J Immunol. 2015 Aug 1;195(3):892-900.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD226 mAb against human CD226 (AA: extra 19-254) recombinant protein. (Expected MW is 52.7 kDa)
Western Blot
Figure 3:Western blot analysis using CD226 mAb against HEK293-6e (1) and CD226 (AA: extra 19-254)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of THP-1 cells using CD226 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CD226 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CD226 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.