CD223 Primary Antibody

Item Information
Catalog #
Size
Price
Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.

Product Overview
Entrez GenelD
3902
Aliases
LAG3
Clone#
4H6A3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD223 (AA: extra 66-199) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Immunol. 2015 Apr 15;194(8):3873-82.2.Immunol Lett. 2013 Feb;150(1-2):116-22.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD223 mAb against human CD223 (AA: extra 66-199) recombinant protein. (Expected MW is 40.4 kDa)
Figure 2:Western blot analysis using CD223 mAb against human CD223 (AA: extra 66-199) recombinant protein. (Expected MW is 40.4 kDa)
Western Blot
Figure 3:Western blot analysis using CD223 mAb against HEK293 (1) and CD223 (AA: extra 66-199)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD223 mAb against HEK293 (1) and CD223 (AA: extra 66-199)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD223 mouse mAb against Raji (1), Ramos (2), and MOLT4 (3) cell lysate.
Figure 4:Western blot analysis using CD223 mouse mAb against Raji (1), Ramos (2), and MOLT4 (3) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Jurkat cells using CD223 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using CD223 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.