CD207 Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity. [provided by RefSeq, Aug 2010]
Product Overview
Entrez GenelD
50489
Aliases
CLEC4K
Clone#
2E1B2
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CD207 (AA: 65-328) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Immunol Cell Biol. 2015 Oct;93(9):815-24. 2.J Immunol. 2011 Feb 1;186(3):1377-83.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD207 mAb against human CD207 (AA: 65-328) recombinant protein. (Expected MW is 32.7 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD207 mAb against HEK293-6e (1) and CD207 (AA: 65-328)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of HEPG2 cells using CD207 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Sw-620 cells using CD207 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded Skin tissue using CD207 mouse mAb with DAB staining.
IMMUNOFLUORESCENCE ANALYSIS
Figure 7: Immunofluorescence analysis of Hela cells using CD207 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.