CD207 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity.
Product Overview
Entrez GenelD
50489
Aliases
CLEC4K
Clone#
1C5A1
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD207 (AA: extra 155-328) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Immunol Cell Biol. 2015 Oct;93(9):815-24.
2.J Immunol. 2011 Feb 1;186(3):1377-83.
2.J Immunol. 2011 Feb 1;186(3):1377-83.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD207 mAb against human CD207 (AA: extra 155-328) recombinant protein. (Expected MW is 45.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD207 mAb against HEK293 (1) and CD207 (AA: extra 155-328)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of K562 cells using CD207 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.