Mouse Monoclonal Antibody to CD206
Item Information
Catalog #
Size
Price
Description
The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015]
Product Overview
Entrez GenelD
4360
Aliases
MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
Clone#
1E12A7
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD206 (AA: extra(19-218)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
References
1,Gastroenterology. 2020 May;158(6):1745-1761.2,Sci Rep. 2019 Dec 10;9(1):18750.
Product Image
Western Blot
![Figure 1:Western blot analysis using CD206 mAb against human CD206 (AA: extra(19-218)) recombinant protein. (Expected MW is 25.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5d56799a8aa166e0dba80635b900c460909067e3da8b2dd7d43223ef840b6b26_51c1e9f8-0986-4824-a91f-5a42af705911.jpg?v=1666705468)
Figure 1:Western blot analysis using CD206 mAb against human CD206 (AA: extra(19-218)) recombinant protein. (Expected MW is 25.7 kDa)
Western Blot
![Figure 2:Western blot analysis using CD206 mAb against HEK293 (1) and CD206 (AA: extra(19-218))-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c85a1280a5d23e1838e8ceaff0483d635e5d542bf79aeb2187bc7fd490d92bc8_8a61da39-2c32-4d34-9bc6-b8f4874b6a8a.jpg?v=1666705468)
Figure 2:Western blot analysis using CD206 mAb against HEK293 (1) and CD206 (AA: extra(19-218))-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric analysis
![Figure 3:Flow cytometric analysis of MOLT4 cells using CD206 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1b37f7b15a1b179a45b02024cf23956f89130c365d55622287306fb522a96b2b_50b2d5db-6155-4fbd-9f9a-265b1e2bdbd6.jpg?v=1666705468)
Figure 3:Flow cytometric analysis of MOLT4 cells using CD206 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of U937 cells using CD206 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e4c6025a25f07e9a2fb0439fc2a8c68ee42c2d134bd33c82d4636ec186585605_f4101b97-f380-41ba-b59d-56b31517a2a1.jpg?v=1666705468)
Figure 4:Flow cytometric analysis of U937 cells using CD206 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
![Figure 5:Immunofluorescence analysis of Hela cells using CD206 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/39d359a1ee85dec17469c0443d7b81a9ad03103a0aefa493de415ea9e9e6b3e3_5d419e2e-c1e0-49ba-a704-8ad240e0bb57.jpg?v=1666705468)
Figure 5:Immunofluorescence analysis of Hela cells using CD206 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.