CD204 Primary Antibody

Item Information
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Description

This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.

Product Overview
Entrez GenelD
4481
Aliases
MSR1; SRA; SR-A; SR-AI; phSR1; phSR2; SCARA1; SR-AII; SR-AIII
Clone#
5B8H4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD204 (AA: extra 275-451) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Asian Pac J Cancer Prev. 2015;16(13):5407-13.
2.Cancer Sci. 2013 Aug;104(8):1112-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD204 mAb against human CD204 (AA: extra 275-451) recombinant protein. (Expected MW is 44.3 kDa)
Figure 2:Western blot analysis using CD204 mAb against human CD204 (AA: extra 275-451) recombinant protein. (Expected MW is 44.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD204 mAb against HEK293 (1) and CD204 (AA: extra 275-451)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD204 mAb against HEK293 (1) and CD204 (AA: extra 275-451)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD204 mouse mAb against PC-2 (1), A549 (2), HepG2 (3), L-02 (4), and PANC-1 (5) cell lysate.
Figure 4:Western blot analysis using CD204 mouse mAb against PC-2 (1), A549 (2), HepG2 (3), L-02 (4), and PANC-1 (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CD204 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HL-60 cells using CD204 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.