Mouse Monoclonal Antibody to CD200
Item Information
Catalog #
Size
Price
Description
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Overview
Entrez GenelD
4345
Aliases
MRC; MOX1; MOX2; OX-2
Clone#
3G1C9
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CD200 (AA:Extra(31-232)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Cancer Immunol Immunother. 2020 Nov;69(11):2333-2343.2,PLoS One. 2020 Mar 23;15(3):e0230621.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD200 mAb against human CD200 (AA:Extra(31-232)) recombinant protein. (Expected MW is 25 kDa)
Western Blot
Figure 3:Western blot analysis using CD200 mAb against HEK293-6e (1) and human CD200 (AA:Extra(31-232))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Raji cells using CD200 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of K562 cells using CD200 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of THP-1 cells using CD200 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using CD200 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.