Mouse Monoclonal Antibody to CD1D

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Description

This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Two transcript variants encoding different isoforms have been found for this gene.

Product Overview
Entrez GenelD
912
Aliases
R3; CD1A; R3G1
Clone#
3A6G3
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human CD1D (AA: extra 20-301) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Med. 2019 Nov;8(16):7065-7073. 2.Brain Pathol2020 Jan;30(1):26-35.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD1D mAb against human CD1D (AA: extra 20-301) recombinant protein. (Expected MW is 31.9 kDa)
Figure 2:Western blot analysis using CD1D mAb against human CD1D (AA: extra 20-301) recombinant protein. (Expected MW is 31.9 kDa)
Western Blot
Figure 3:Western blot analysis using CD1D mAb against HEK293-6e (1) and CD1D (AA: extra 20-301)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD1D mAb against HEK293-6e (1) and CD1D (AA: extra 20-301)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using CD1D mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using CD1D mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 32162

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