CD1B Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.
Product Overview
Entrez GenelD
910
Aliases
R1; CD1; CD1A
Clone#
1D6B7
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD1B (AA: extra 18-303) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):380-5.
2.J Exp Med. 2011 Aug 29;208(9):1741-7.
2.J Exp Med. 2011 Aug 29;208(9):1741-7.
Product Image
Western Blot
Figure 1:Western blot analysis using CD1B mAb against human CD1B (AA: extra 18-303) recombinant protein. (Expected MW is 57.1 kDa)
Western Blot
Figure 2:Western blot analysis using CD1B mAb against HEK293 (1) and CD1B (AA: extra 18-303)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3:Flow cytometric analysis of Raji cells using CD1B mouse mAb (green) and negative control (red).
Elisa
Figure 4:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
For Research Use Only. Not for use in diagnostic procedures.