Mouse Monoclonal Antibody to CD1A

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Product Overview

Entrez GenelD

909

Aliases

R4; T6; CD1; FCB6; HTA1

Clone#

5B9C3

Host / Isotype

Mouse / IgG2a

Immunogen

Purified recombinant fragment of human CD1A (AA: 17-116) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Mol Immunol. 2018 Nov;103:200-208.2,Sci Immunol. 2017 Dec 22;2(18):eaan5918.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD1A mAb against human CD1A (AA: 17-116) recombinant protein. (Expected MW is 39.7 kDa)

Western Blot

Figure 3:Western blot analysis using CD1A mouse mAb against Hela (1), HepG2 (2), HEK293 (3),MOLT4 (4),K562 (5),HEK293-6e (6),Cos-7 (7),and NIH/3T3 (8) cell lysate.

Flow cytometric analysis

Figure 4:Flow cytometric analysis of MOLT4 cells using CD1A mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 5:Flow cytometric analysis of HL-60 cells using CD1A mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 6:Flow cytometric analysis of Jukrat cells using CD1A mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 7:Flow cytometric analysis of K562 cells using CD1A mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD1A mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD1A mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 10:Immunofluorescence analysis of Hela cells using CD1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.