CD196 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.
Product Overview
Entrez GenelD
1235
Aliases
CCR6; BN-1; DCR2; DRY6; CCR-6; CKRL3; GPR29; CKR-L3; CMKBR6; GPRCY4; STRL22; CC-CKR-6; C-C CKR-6
Clone#
7C6D9
Host / Isotype
Mouse / Mouse IgG2a
Immunogen
Purified recombinant fragment of human CD196 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Cancer Res Treat. 2018 Oct;50(4):1203-1213. 2.Clin Rheumatol. 2017 Jun;36(6):1453-1456.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD196 mAb against human CD196 recombinant protein. (Expected MW is 39.3 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD196 mAb against HEK293 (1) and CD196-hIgGFc transfected HEK293 (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using CD196 mouse mAb against K562 (1), THP-1 (2), and HL-60 (3) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of THP-1 cells using CD196 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.