CD19 Primary Antibody
Item Information
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Size
Price
Description
The CD19 antigen (95kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.
Product Overview
Entrez GenelD
930
Aliases
B4; MGC12802
Clone#
2E2
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD19 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Rie, M.A. de, J. of Immunol. Methods, 1987. 102: 187.
2. Rie, M.A. de, Leukaemia Research, 1988. 12: 135.
2. Rie, M.A. de, Leukaemia Research, 1988. 12: 135.
Product Image
Flow cytometric
Figure 1: Flow cytometric analysis of Raji cells using CD19 mouse mAb (green) and negative control (purple).
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of HL-60(left) and K562 (right) cells using CD19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.