CD19 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesB4; MGC12802
ProductOrderC
Clone#2E2
Entrez GenelD930
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human CD19 expressed in E. Coli.
MW61kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
N
Catalog#: 30039
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Images
Flow cytometric
Figure 1: Flow cytometric analysis of Raji cells using CD19 mouse mAb (green) and negative control (purple).
Figure 1: Flow cytometric analysis of Raji cells using CD19 mouse mAb (green) and negative control (purple).
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of HL-60(left) and K562 (right) cells using CD19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Immunofluorescence analysis of HL-60(left) and K562 (right) cells using CD19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Product Overview
Description

The CD19 antigen (95kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.

References (references)
References (references)1. Rie, M.A. de, J. of Immunol. Methods, 1987. 102: 187.
2. Rie, M.A. de, Leukaemia Research, 1988. 12: 135.