CD180
Item Information
Catalog #
Size
Price
Description
CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria
Product Overview
Entrez GenelD
4064
Aliases
LY64; Ly78; RP105
Clone#
2A4D6
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD180 (AA: extra 24-185) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Emerg Microbes Infect. 2018 Mar 7;7(1):19.
2.Reproduction. 2017 Dec;154(6):735-744.
2.Reproduction. 2017 Dec;154(6):735-744.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32319ea.jpg?v=1669029576)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD180 mAb against human CD180 (AA: extra 24-185) recombinant protein. (Expected MW is 44.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32319rwb.jpg?v=1669029576)
Figure 2:Western blot analysis using CD180 mAb against human CD180 (AA: extra 24-185) recombinant protein. (Expected MW is 44.7 kDa)
Western Blot
![Figure 3:Western blot analysis using CD180 mAb against HEK293-6e (1) and CD180 (AA: extra 24-185)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32319twb.jpg?v=1669029576)
Figure 3:Western blot analysis using CD180 mAb against HEK293-6e (1) and CD180 (AA: extra 24-185)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
![Figure 4:Flow cytometric analysis of THP-1 cells using CD180 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32319fcm.jpg?v=1669029576)
Figure 4:Flow cytometric analysis of THP-1 cells using CD180 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CD180 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32319ihc.jpg?v=1669029576)
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CD180 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD180 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32319-1ihc.jpg?v=1669029576)
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD180 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.