CD18 Primary Antibody
Item Information
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Size
Price
Description
CD18, also known as ITGB2 (integrin beta chain beta 2). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. CD18 is expressed by most leucocytes. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
Product Overview
Entrez GenelD
3689
Aliases
LAD; MF17; LCAMB; LFA-1; ITGB2
Clone#
10E12
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of CD18 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Microcirculation. 2008 Aug;15(6):555-67.
2. Mol Immunol. 2008 Feb;45(3):709-18.
3. J Biol Chem. 2007 Aug 17;282(33):24310-9.
2. Mol Immunol. 2008 Feb;45(3):709-18.
3. J Biol Chem. 2007 Aug 17;282(33):24310-9.
Product Image
Immunofluorescence analysis
Figure 1: Confocal Immunofluorescence analysis of HL60 cells using CD18 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using CD18 mouse mAb (red). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.