Mouse Monoclonal Antibody to CD178
Item Information
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Price
Description
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.
Product Overview
Entrez GenelD
356
Aliases
FASLG; APTL; FASL; CD95L; ALPS1B; CD95-L; TNFSF6; TNLG1A; APT1LG1
Clone#
2A6E12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD178 (AA: extra 103-281) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Cancer Res. 2019 Dec;17(12):2537-2548. 2.Cell Death Dis. 2018 Jun 11;9(6):695.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 4:Western blot analysis using CD178 mAb against human CD178 (AA: extra 103-281) recombinant protein. (Expected MW is 46.4 kDa)
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using CD178 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Western Blot
Figure 5:Western blot analysis using CD178 mAb against HEK293-6e (1) and CD178 (AA: extra 103-281)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using CD178 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.