CD177 Primary Antibody
Item Information
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Size
Price
Description
This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.
Product Overview
Entrez GenelD
57126
Aliases
NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
Clone#
2F2C1
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Immunol Lett. 2016 Oct;178:122-30.
2.Immunohematology. 2015;31(3):128-9.
2.Immunohematology. 2015;31(3):128-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD177 mAb against human CD177 (AA: extra 22-161) recombinant protein. (Expected MW is 41.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD177 mAb against HEK293 (1) and CD177 (AA: extra 22-161)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD177 mouse mAb against SPC-A-1 (1), SK-MES-1 (2), HepG2 (3), HL-60 (4), and PC-3 (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CD177 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.