CD172G Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Overview

Entrez GenelD

55423

Aliases

SIRPG; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma

Clone#

4F8C10

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CD172G (AA: extra 29-360) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. BMC Struct Biol. 2013 Jul 4;13:13.
2. J Immunol. 2004 Aug 15;173(4):2562-70.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD172G mAb against human CD172G (AA: extra 29-360) recombinant protein. (Expected MW is 62.6 kDa)

Western Blot

Figure 3:Western blot analysis using CD172G mAb against HEK293 (1) and CD172G (AA: extra 29-360)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CD172G mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric