Mouse Monoclonal Antibody to CD172b
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
10326
Aliases
SIRPB1; SIRP-BETA-1
Clone#
1B1G4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD172b (AA: extra 72-309) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Genes Brain Behav. 2014 Sep;13(7):653-62. 2.J Biol Chem. 2005 Oct 28;280(43):36132-40.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD172b mAb against human CD172b (AA: extra 72-309) recombinant protein. (Expected MW is 28.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD172b mAb against HEK293-6e (1) and CD172b (AA: extra 72-309)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using CD172b mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of K562 cells using CD172b mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CD172b mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CD172b mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.