CD170 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.
Product Overview
Entrez GenelD
8778
Aliases
SIGLEC5; OBBP2; CD33L2; OB-BP2; SIGLEC-5
Clone#
3F5A3
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD170 (AA: extra 17-230) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Mol Med (Berl). 2013 Feb;91(2):261-70.
2.Protein Expr Purif. 2013 Apr;88(2):183-9.
2.Protein Expr Purif. 2013 Apr;88(2):183-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD170 mAb against human CD170 (AA: extra 17-230) recombinant protein. (Expected MW is 50.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD170 mAb against HEK293 (1) and CD170 (AA: extra 17-230)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD170 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.