CD16B Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1.
Product Overview
Entrez GenelD
2215
Aliases
FCGR3B; CD16; FCG3; CD16A; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb
Clone#
8F1C10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD16B (AA: 17-200) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Lupus. 2016 Oct;25(11):1237-43.2.Arthritis Res Ther. 2012 Feb 7;14(1):R28.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD16B mAb against human CD16B (AA: 17-200) recombinant protein. (Expected MW is 46.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD16B mAb against HEK293 (1) and CD16B (AA: 17-200)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using CD16B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.