CD169 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined.
Product Overview
Entrez GenelD
6614
Aliases
SIGLEC1; SN; SIGLEC-1; dJ1009E24.1
Clone#
2A4D1
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD169 (AA: extra 20-197) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Virol. 2017 Oct 13;91(21). pii: e00972-17.
2.Cancer Sci. 2017 Mar;108(3):290-295.
2.Cancer Sci. 2017 Mar;108(3):290-295.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD169 mAb against human CD169 (AA: extra 20-197) recombinant protein. (Expected MW is 45.9 kDa)
Western Blot
Figure 3:Western blot analysis using CD169 mAb against HEK293 (1) and CD169 (AA: extra 20-197)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD169 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using CD169 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.