CD15 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]
Product Overview
Entrez GenelD
2526
Aliases
LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Clone#
6C5A3
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CD15 (AA: 1-147) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Int J Mol Sci. 2019 Apr 3;20(7):1651.2,J Cell Biochem. 2018 Aug;119(8):6442-6451.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD15 mAb against human CD15 (AA: 1-147) recombinant protein. (Expected MW is 34.7 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD15 mAb against HEK293-6e (1) and CD15 (AA: 1-147)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of THP-1 cells using CD15 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Jurkat cells using CD15 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.