CD148 Primary Antibody

Item Information
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Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Overview
Entrez GenelD
5795
Aliases
PTPRJ; DEP1; SCC1; HPTPeta; R-PTP-ETA
Clone#
1D2C11
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD148 (AA: extra 36-210) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncogene. 2015 Oct 29;34(44):5536-47.
2.J Neurooncol. 2015 May;122(3):451-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD148 mAb against human CD148 (AA: extra 36-210) recombinant protein. (Expected MW is 44.3 kDa)
Figure 2:Western blot analysis using CD148 mAb against human CD148 (AA: extra 36-210) recombinant protein. (Expected MW is 44.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD148 mAb against HEK293 (1) and CD148 (AA: extra 36-210)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD148 mAb against HEK293 (1) and CD148 (AA: extra 36-210)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD148 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HL-60 cells using CD148 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.