Mouse Monoclonal Antibody to CD147
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
682
Aliases
BSG; OK; 5F7; TCSF; EMMPRIN
Clone#
2D3A9
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD147 (AA: extra 138-323) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncotarget. 2016 Feb 2;7(5):5613-29. 2.J Biochem. 2016 May;159(5):481-90.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e8c56961d442a4c7a2d1fde96e25fd9ff171fff73653ec95439b67fb6f9965a9.jpg?v=1666705457)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD147 mAb against human CD147 (AA: extra 138-323) recombinant protein. (Expected MW is 46.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a3358c863cfe882c5be0203b6c06fc8b397343bf35d352c39f82e16b9fbacca4.jpg?v=1666705457)
Figure 2:Western blot analysis using CD147 mAb against human CD147 (AA: extra 138-323) recombinant protein. (Expected MW is 46.3 kDa)
Western Blot
![Figure 3:Western blot analysis using CD147 mAb against HEK293-6e (1) and CD147 (AA: extra 138-323)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/37216550a7b6e5398cb92486581541215c79b9c234cf8edf7ec0b85e05f7082a.jpg?v=1666705457)
Figure 3:Western blot analysis using CD147 mAb against HEK293-6e (1) and CD147 (AA: extra 138-323)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
![Figure 4:Immunofluorescence analysis of Hela cells using CD147 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/cce8999a5e0cc6734c4c8c615ad5b7734ef468bec0e10da796890b1c14f0257b.jpg?v=1666705457)
Figure 4:Immunofluorescence analysis of Hela cells using CD147 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of THP-1 cells using CD147 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/84a19016c8c2b60f91bcb10440c3864ad223ef825164eb06ae4400b04eb28e56.jpg?v=1666705457)
Figure 5:Flow cytometric analysis of THP-1 cells using CD147 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD147 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d8e88afa105fc645372aef6d236327a35a82457ccf711b1fc34b154993431824.jpg?v=1666705457)
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD147 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD147 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9a7444c31f5f05112ec319e7c8e1103403af9936eed92ec544e58060ccf2f20a.jpg?v=1666705457)
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD147 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.