CD140a Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Product Overview
Entrez GenelD
5156
Aliases
PDGFRA; PDGFR2; PDGFR-2
Clone#
5B11G5
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD140a (AA: extra 179-361) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.World J Gastroenterol. 2016 Mar 28;22(12):3335-40.
2.Gene Expr. 2015;16(3):109-27.
2.Gene Expr. 2015;16(3):109-27.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD140a mAb against human CD140a (AA: extra 179-361) recombinant protein. (Expected MW is 46.5 kDa)
Western Blot
Figure 3:Western blot analysis using CD140a mAb against HEK293 (1) and CD140a (AA: extra 179-361)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Raji cells using CD140a mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD140a mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.