CD14 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
ProductOrderC
Clone#4B4F12
Entrez GenelD929
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli.
MW40kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
20-214
Catalog#: 30590
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Images
Western Blot
Figure 1: Western blot analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)
Figure 1: Western blot analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)
Western Blot
Figure 2: Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.
Figure 3: Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD14 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD14 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThe protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.

References (references)
References (references)1. J Immunol. 2012 Dec 15;189(12):5729-44.
2. Iran J Immunol. 2011 Jun;8(2):111-9.