CD14 Primary Antibody
Item Information
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Price
Description
CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
Product Overview
Entrez GenelD
929
Aliases
CD14
Clone#
5A3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD14 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Mammary Gland Biol Neoplasia 2000, 5 : 227-241.
2. J Mammary Gland Biol Neoplasia 2000, 5 : 165-185.
2. J Mammary Gland Biol Neoplasia 2000, 5 : 165-185.
Product Image
Immunohistochemical analysis
Figure 1: Immunohistochemical analysis of paraffin-embedded human lymphnode, showing membrane localization using CD14 mouse mAb with DAB staining.
Flow cytometric
Figure 2: Flow cytometric analysis of human PBMC using CD14 mouse mAb.
For Research Use Only. Not for use in diagnostic procedures.