CD14 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
Product Overview
Entrez GenelD
929
Clone#
1D1F8
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CD14 (AA: 230-345) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem. 2018 Nov 9;293(45):17631-17645. 2.Biochim Biophys Acta Mol Basis Dis. 2018 Dec;1864(12):3559-3567.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD14 mAb against human CD14 (AA: 230-345) recombinant protein. (Expected MW is 27.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 230-345)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using CD14 mouse mAb (green) and negative control (red).
Immunohistochemical Analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD14 mouse mAb with DAB staining.
Immunohistochemical Analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD14 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.