CD137 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.

Product Overview

Entrez GenelD

3604

Aliases

TNFRSF9; ILA; 4-1BB; CDw137

Clone#

2C6B4

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human CD137 (AA: 214-255) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

FCM (Flow Cytometry)

1/200-1/400

ELISA

1/10000

References

1.Blood. 2019 Oct 31;134(18):1510-1516. 2.Cancer Commun (Lond). 2019 Jul 9;39(1):41.

Product Image

ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Figure 2: Western blot analysis using CD137 mAb against human CD137 (AA: 214-255) recombinant protein. (Expected MW is 30.9 kDa)

WESTERN BLOT

Figure 3: Western blot analysis using CD137 mAb against HEK293-6e (1) and CD137 (AA: 214-255)-hIgGFc transfected HEK293-6e (2) cell lysate.

WESTERN BLOT

Figure 4: Western blot analysis using CD137 mouse mAb against HepG2 (1), Jurkat (2), MOLT4 (3), HL-60 (4), THP-1 (5), Ramos (6), K562 (7), and Raji (8) cell lysate.

FLOW CYTOMETRY