CD130 Primary Antibody

Item Information
Catalog # Size/Concentration Price
31686 100μg $341.00
Specification
AliasesIL6ST; GP130; CDW130; IL-6RB
Clone#8D4C5
Entrez GeneID3572
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CD130 (AA: extra 73-231) expressed in E. Coli.
MW103.5kDa
Species ReactivityHuman, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
Extra (73-231)
Catalog
31686
$341.00
-
+

Contact us to order

Images

Elisa

Cell Culture Products

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products

Figure 2:Western blot analysis using CD130 mAb against human CD130 (AA: extra 73-231) recombinant protein. (Expected MW is 43.8 kDa)

Western Blot

Cell Culture Products

Figure 3:Western blot analysis using CD130 mAb against HEK293 (1) and CD130 (AA: extra 73-231)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Cell Culture Products

Figure 4:Western blot analysis using CD130 mouse mAb against COS7 (1) cell lysate.

Immunofluorescence analysis

Cell Culture Products

Figure 5:Immunofluorescence analysis of Hela cells using CD130 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Cell Culture Products

Figure 6:Flow cytometric analysis of HL-60 cells using CD130 mouse mAb (green) and negative control (red).

Product Overview
Description

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]

References (references)
References (references) 1.J Exp Med. 2017 Sep 4;214(9):2547-2562.
2.Biochem Biophys Res Commun. 2016 Feb 19;470(4):870-6.

Please view FAQs here