CD127 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found.
Product Overview
Entrez GenelD
3575
Aliases
IL7R; ILRA; IL7RA; CDW127; IL-7R-alpha
Clone#
3F5D9
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD127 (AA: extra 21-239) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Clin Immunol. 2017 Jan;37(1):42-50.
2.Blood. 2016 Jul 28;128(4):473-8.
2.Blood. 2016 Jul 28;128(4):473-8.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD127 mAb against human CD127 (AA: extra 21-239) recombinant protein. (Expected MW is 51 kDa)
Western Blot
Figure 3:Western blot analysis using CD127 mAb against HEK293 (1) and CD127 (AA: extra 21-239)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD127 mouse mAb against Hela (1), HL-60 (2), and U937 (3) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CD127 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.