Mouse Monoclonal Antibody to CD126

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesIL6Q; gp80; CD126; HIES5; IL-6R; IL6RA; IL6RQ; IL-1Ra; IL-6RA; IL6QTL; IL-6R-1
Clone#8F7G6
Entrez GenelD3570
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CD126 (AA: EXTRA 20-177) expressed in E. Coli.
MW52kDa
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
EXTRA 20-177
Catalog#: 32241
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD126 mAb against human CD126 (AA:20-177) recombinant protein. (Expected MW is 20.3 kDa)
Figure 2:Western blot analysis using CD126 mAb against human CD126 (AA:20-177) recombinant protein. (Expected MW is 20.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD126 mAb against HEK293 (1) and CD126 (AA:20-177)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD126 mAb against HEK293 (1) and CD126 (AA:20-177)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD126 mouse mAb against Jurkat (1), MOLT4 (2), Raw264.7 (3)and THP-1 (4) cell lysate.
Figure 4:Western blot analysis using CD126 mouse mAb against Jurkat (1), MOLT4 (2), Raw264.7 (3)and THP-1 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using CD126 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using CD126 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using CD126 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using CD126 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of K562 cells using CD126 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of K562 cells using CD126 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 8:Flow cytometric analysis of THP-1 cells using CD126 mouse mAb (green) and negative control (red).
Figure 8:Flow cytometric analysis of THP-1 cells using CD126 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 9:Flow cytometric analysis of U937 cells using CD126 mouse mAb (green) and negative control (red).
Figure 9:Flow cytometric analysis of U937 cells using CD126 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD126 mouse mAb with DAB staining.
Figure 10:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD126 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD126 mouse mAb with DAB staining.
Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD126 mouse mAb with DAB staining.
Product Overview
Description

This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been identified in this gene. A pseudogene of this gene is found on chromosome 9. [provided by RefSeq, Aug 2020]

References (references)
References (references)1.Medicine (Baltimore).2020 Dec 11;99(50):e23659.2.Biomed Res Int.2021 Jan 28;2021:2615059.