Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD126 mAb against human CD126 (AA:20-177) recombinant protein. (Expected MW is 20.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD126 mAb against HEK293 (1) and CD126 (AA:20-177)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD126 mouse mAb against Jurkat (1), MOLT4 (2), Raw264.7 (3)and THP-1 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using CD126 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using CD126 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of K562 cells using CD126 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 8:Flow cytometric analysis of THP-1 cells using CD126 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 9:Flow cytometric analysis of U937 cells using CD126 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD126 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD126 mouse mAb with DAB staining.
