CD120B Primary Antibody

Item Information
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Size
Price
Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.

Product Overview
Entrez GenelD
7133
Aliases
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Clone#
7G8B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD120B (AA: extra 23-257) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Clin Exp Immunol. 2016 Aug;185(2):263-70.
2.Cancer Immunol Immunother. 2015 Nov;64(11):1475-85.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD120B mAb against human CD120B (AA: extra 23-257) recombinant protein. (Expected MW is 51 kDa)
Figure 2:Western blot analysis using CD120B mAb against human CD120B (AA: extra 23-257) recombinant protein. (Expected MW is 51 kDa)
Western Blot
Figure 3:Western blot analysis using CD120B mAb against HEK293 (1) and CD120B (AA: extra 23-257)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD120B mAb against HEK293 (1) and CD120B (AA: extra 23-257)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Ramos cells using CD120B mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Ramos cells using CD120B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.