CD11D Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene belongs to the beta-2 integrin family of membrane glycoproteins, which are are composed of non-covalently linked alpha and beta subunits to form a heterodimer. It encodes the alpha subunit of the cell surface heterodimers and is involved in the activation and adhesion functions of leukocytes. The gene is located about 11kb downstream of the integrin subunit alpha X gene, another member of the integrin family. It is expressed in the tissue and circulating myeloid leukocytes. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
3681
Aliases
ITGAD; ADB2
Clone#
3D7A6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD11D (AA: extra 18-172) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Leukoc Biol. 2017 Apr;101(4):1029-1035.
2.J Neuroinflammation. 2013 Feb 15;10:26.
2.J Neuroinflammation. 2013 Feb 15;10:26.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD11D mAb against human CD11D (AA: extra 18-172) recombinant protein. (Expected MW is 42.4 kDa)
Western Blot
Figure 3:Western blot analysis using CD11D mAb against HEK293 (1) and CD11D (AA: extra 18-172)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD11D mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.