CD11a Primary Antibody

Item Information
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Description

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.

Product Overview
Entrez GenelD
3683
Aliases
ITGAL; LFA-1; LFA1A
Clone#
5G7C5
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD11a (AA: extra 26-194) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Clin Exp Pathol. 2015 Dec 1;8(12):15642-51.
2.Cytometry B Clin Cytom. 2013 Nov-Dec;84(6):370-8.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD11a mAb against human CD11a (AA: extra 26-194) recombinant protein. (Expected MW is 44.4 kDa)
Figure 2:Western blot analysis using CD11a mAb against human CD11a (AA: extra 26-194) recombinant protein. (Expected MW is 44.4 kDa)
Western Blot
Figure 3:Western blot analysis using CD11a mAb against HEK293 (1) and CD11a (AA: extra 26-194)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD11a mAb against HEK293 (1) and CD11a (AA: extra 26-194)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD11a mouse mAb against HL-60 (1), A549 (2), and SW620 (3) cell lysate.
Figure 4:Western blot analysis using CD11a mouse mAb against HL-60 (1), A549 (2), and SW620 (3) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CD11a mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HL-60 cells using CD11a mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using CD11a mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using CD11a mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD11a mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD11a mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.