CD114 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.
Product Overview
Entrez GenelD
1441
Aliases
CSF3R; SCN7; GCSFR
Clone#
8F8B12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD114 (AA: extra 25-187) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Clin Cancer Res. 2016 Feb 1;22(3):757-64.
2.World J Gastroenterol. 2014 Jan 28;20(4):1074-8.
2.World J Gastroenterol. 2014 Jan 28;20(4):1074-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD114 mAb against human CD114 (AA: extra 25-187) recombinant protein. (Expected MW is 46.5 kDa)
Western Blot
Figure 3:Western blot analysis using CD114 mAb against HEK293 (1) and CD114 (AA: extra 25-187)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD114 mouse mAb against A431 (1), K562 (2), THP-1 (3), MOLT4 (4), and Jurkat (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Ramos cells using CD114 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD114 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.