Mouse Monoclonal Antibody to CD109
Item Information
Catalog #
Size
Price
Description
This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
135228
Aliases
p180; r150; CPAMD7
Clone#
3B5G6
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD109 (AA: extra 1274-1421) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/20 - 1/100
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Sci. 2020 May;111(5):1652-1662. 2.Diagn Pathol. 2015 Aug 7;10:137.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/72841314af3a36ef2d14a940e58531ff38b8001fc1b145f931ecb844ed85a0a6.jpg?v=1666705724)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6a8ca6c28dae10650f7061d2161cb5beaa2e8fd502ab762d5f474ecf27e56276.jpg?v=1666705724)
Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)
Western Blot
![Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/44650fe0482f5c70240f5695d52987ed31d9b108e2a7dc16a942d736f5d26b1c.jpg?v=1666705724)
Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
![Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e1f2f5682cc0e59fc4c9a6101ed85b6c8440753987b65482564e0de142d4ad3d.jpg?v=1666705724)
Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/44ff3f79b01c6c30a4086b80c03e874ffa1bd9d834ac99cffb3241461aee11f8.jpg?v=1666705724)
Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded lung tissues using CD109 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/334e46af7239ee2437a4249228cc8b32422c9c6bfe129b594e5ed46d0820938d.jpg?v=1666705724)
Figure 6:Immunohistochemical analysis of paraffin-embedded lung tissues using CD109 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded stomach tissues using CD109 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b8febb69da6df4cb142f0376f20ac35a30569e99cd59c308bdd764e60ec67d22.jpg?v=1666705724)
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach tissues using CD109 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.