CD107b Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.
Product Overview
Entrez GenelD
3920
Aliases
LAMPB; LAMP2; LAMP-2; LGP-96; LGP110
Clone#
8E2F2
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human CD107b (AA: extra 29-168) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Cell Biochem. 2014 Jan;385(1-2):1-6.
2.Rheumatology (Oxford). 2013 Sep;52(9):1592-8.
2.Rheumatology (Oxford). 2013 Sep;52(9):1592-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD107b mAb against human CD107b (AA: extra 29-168) recombinant protein. (Expected MW is 41.4 kDa)
Western Blot
Figure 3:Western blot analysis using CD107b mAb against HEK293 (1) and CD107b (AA: extra 29-168)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD107b mouse mAb against U937 (1), HepG2 (2), RAW264.7 (3), NIH3T3 (4), C6 (5), and T47D (6) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Ramos cells using CD107b mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CD107b mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.