Mouse Monoclonal Antibody to CD105
Item Information
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Description
This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
2022
Aliases
ENG; END; HHT1; ORW1
Clone#
4B2A1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD105 (AA: extra 342-586) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep. 2019 Dec 4;9(1):18337. 2.Indian J Pathol Microbiol. 2019 Apr-Jun;62(2):239-243.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD105 mAb against human CD105 (AA: extra 342-586) recombinant protein. (Expected MW is 42.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD105 mAb against HEK293-6e (1) and CD105 (AA: extra 342-586)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD105 mouse mAb against HUVEC (1), HUVE-12 (2), SH-SY5Y (3), and HEK293 (4) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using CD105 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum tissues using CD105 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CD105 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.