CD100 Primary Antibody
Item Information
Catalog #
Size
Price
Description
SEMA4D (Semaphorin 4D) is a Protein Coding gene. Diseases associated with SEMA4D include Hemorrhagic Fever With Renal Syndrome. Among its related pathways are Guidance Cues and Growth Cone Motility and Developmental Biology. GO annotations related to this gene include receptor binding and transmembrane signaling receptor activity. An important paralog of this gene is SEMA4B.
Product Overview
Entrez GenelD
10507
Aliases
SEMA4D; SEMAJ; coll-4; C9orf164; M-sema-G
Clone#
5H6E3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD100 (AA: extra 590-734) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2016 Feb 24;11(2):e0150151.
2.Microvasc Res. 2014 May;93:1-8.
2.Microvasc Res. 2014 May;93:1-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD100 mAb against human CD100 (AA: extra 590-734) recombinant protein. (Expected MW is 43.2 kDa)
Western Blot
Figure 3:Western blot analysis using CD100 mAb against HEK293 (1) and CD100 (AA: extra 590-734)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of K562 cells using CD100 mouse mAb (green) and negative control (red).
Flow cytometric
Figure 5:Flow cytometric analysis of Ramos cells using CD100 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD100 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD100 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.