CD10 Primary Antibody

Item Information
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Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.

Product Overview
Entrez GenelD
4311
Aliases
NEP; SFE; MME; CALLA; CMT2T; SCA43
Clone#
1F11D10
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CD10 (AA: 321-496) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Pathobiology. 2015;82(6):259-63. 2.Asian Pac J Cancer Prev. 2015;16(8):3147-52.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD10 mAb against human CD10 (AA: 321-496) recombinant protein. (Expected MW is 23.2 kDa)
Figure 2:Western blot analysis using CD10 mAb against human CD10 (AA: 321-496) recombinant protein. (Expected MW is 23.2 kDa)
Western Blot
Figure 3:Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 321-496)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 321-496)-hIgGFc transfected HEK293 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.