Mouse Monoclonal Antibody to CCR10

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Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CCR10 is the receptor for CCL27 (SCYA27; MIM 604833); CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (Homey et al., 2002 [PubMed 11821900]).[supplied by OMIM, Mar 2008]

Product Overview
Entrez GenelD
2826
Aliases
GPR2
Clone#
4E4B12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CCR10 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Allergy. 2019 May;74(5):933-943. 2,Int Immunopharmacol. 2017 Oct;51:124-130.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CCR10 mAb against human CCR10 (AA: extra mix) recombinant protein. (Expected MW is 38 kDa)
Figure 2:Western blot analysis using CCR10 mAb against human CCR10 (AA: extra mix) recombinant protein. (Expected MW is 38 kDa)
Western Blot
Figure 3:Western blot analysis using CCR10 mAb against HEK293-6e (1) and CCR10 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CCR10 mAb against HEK293-6e (1) and CCR10 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of HL-60 cells using CCR10 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HL-60 cells using CCR10 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of MOLT4 cells using CCR10 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of MOLT4 cells using CCR10 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of THP-1 cells using CCR10 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of THP-1 cells using CCR10 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.