CCNE1 Primary Antibody

Item Information
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Price
Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Product Overview
Entrez GenelD
898
Aliases
CCNE
Clone#
5F8C5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human CCNE1 (AA: 307-410) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Cancer Res. 2010 Jun 15;70(12):5074-84.
2. Cancer. 2010 Jun 1;116(11):2621-34.
Product Image
Western Blot
Figure 1: Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)
Figure 1: Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)
Western Blot
Figure 2: Western blot analysis using CCNE1 mAb against HEK293 (1) and CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using CCNE1 mAb against HEK293 (1) and CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.
Figure 3: Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.