CCNB3

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCYCB3
Clone#4B4D8
Entrez GenelD85417
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human CCNB3 (AA: 142-363) expressed in E. Coli.
MW158kDa
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
142-363
Catalog#: 32310
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CCNB3 mAb against human CCNB3 (AA: 142-363) recombinant protein. (Expected MW is 28.3 kDa)
Figure 2:Western blot analysis using CCNB3 mAb against human CCNB3 (AA: 142-363) recombinant protein. (Expected MW is 28.3 kDa)
Western Blot
Figure 3:Western blot analysis using CCNB3 mAb against HEK293-6e (1) and CCNB3 (AA: 142-363)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CCNB3 mAb against HEK293-6e (1) and CCNB3 (AA: 142-363)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Hela cells using CCNB3 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using CCNB3 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Jurkat cells using CCNB3 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using CCNB3 mouse mAb (green) and negative control (red).
Product Overview
Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as positive regulators of cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chicken and drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and may be required for proper spindle reorganization and restoration of the interphase nucleus. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

References (references)
References (references)1.Pediatr Blood Cancer. 2020 Apr;67(4):e28151.
2.Am J Surg Pathol. 2017 Dec;41(12):1713-1721.